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KMID : 1094720150200020366
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Biotechnology and Bioprocess Engineering
2015 Volume.20 No. 2 p.366 ~ p.372
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A solid-phase screening method for identification of glycan-binding cells
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Kim Yoon-Woo
Kim Kyoung-Jin
Hwang Cheol-Hwan
Kim Yun-Gon
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Abstract
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Glycan immobilization and epitope recognition on a solid support is useful for functional glycoproteomics and analysis of protein-protein interactions. However, the lack of high-throughput experimental methods for the examination of cell-glycan interactions means that identifying glycan-binding cells remains a challenge. Herein, we describe a tool which enables the screening of glycanbinding cells that specifically recognize the glycan epitopes released from the membrane glycoproteins of cells and tissues. Biotinylated pig kidney N-glycans were immobilized on streptavidin-conjugated solid supports and then incubated with human immune cell lines. U937 cells discriminated between the α-galactose (α-Gal) and non-Gal pig N-glycans on the solid support. This screening strategy could aid exploration of cell-protein or cell-cell interactions.
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KEYWORD
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glycan-binding cells, N-glycan, pig kidney, α-galactose, immobilization
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